The Olympus FluoView FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 an ideal choice for FRAP, FLIP and photoactivation.
It is based on a fully motorized Olympus IX81 inverted microscope.
Exitation lasers and lines:
Multi Argon laser with excitations at: 458, 488, 514nm
HeNe594 laser, with excitation line at: 594 nm
HeNe561 laser, with excitation line at: 561 nm
HeNe633 laser, with excitation line at: 633 nm
LD 405 laser, with excitation line at: 405 n
Applications:
Multi channel fluorescence imaging, 3-D reconstruction (Z-stack), Time-series, FRET (Fluorescence Resonance Energy Transfer), FRAP (Fluorescence Recovery After Photobleaching).
Detection options:
4 individual channels: 3 PMT fluorescent detectors and 1 transmitted light channel allowing viewing fluorescence images on DIC (Diffferential Interference Contrast) images.
A variety of available emission filters offers imaging of wide fluorochromes.
Objectives:
Magnitude |
10 X UPlanSApo |
20 X UPlanSApo |
40X UPlanSApo |
63X UPlanSApo |
NA |
0.40 |
0.75 |
0.90 |
1.35 |
Immersion |
Dry |
Dry |
Dry |
Oil , cover slip |
Reflectors:
Fluorescence Filter cube |
Filter Set
|
Excitation
|
Beam Splitter |
Emission
|
|
None
|
Bright filed |
||||
WB (U-MWB2)
|
Blue Excitation
|
BP 460-490 |
DM505 |
LP 520 |
Alexa 488, GFP, FITC |
WG (U-MWG2)
|
Green Excitation |
BP 510-550 |
DM570 |
LP 590 |
Rhodamine |
WIY (U-MWIY2)
|
Yellow Excitation
|
BP 545-580 |
DM 600 |
LP 610IF |
|
U-MWU2 |
Ultraviolet Excitation |
BP 330-385 |
DM 400 |
LP 420 |
DAPI
|
Analyser module D |
DIC |